复仇者联盟以及随后影响深远的纽约之战，让外星人和超能力者不再是坊间传说的秘密。纽约之战过后，世界格局即将重新洗牌，野心家们则蠢蠢欲动，神盾局面临前所未有的挑战。在此危急关头，一度传闻死于纽约的特工菲尔·科尔森探员（克拉克·格雷格 Clark Gregg 饰）“复活”，吸纳了身怀绝技的格兰特•沃德（布雷特·道顿 Brett Dalton 饰）、梅琳达·梅（温明娜 Ming-Na Wen 饰）、莱奥·菲兹（伊恩·德·卡兹泰克 Iain De Caestecker 饰）、盖玛·西蒙斯（伊丽莎白·亨斯屈奇 Elizabeth Henstridge 饰）以及来自黑客构成的民间团体涨潮组织的斯凯（科洛·本内特 Chloe Bennet 饰）加入进来。 　　他们以一己凡人之力，投入到各种匪夷所思且疑云重重的危险事件的调查中去……After the Battle of New York, the world has changed. It now knows not only about the Avengers, but also the powerful menaces that require those superheroes and more to face them. In response, Phil Coulson of the Strategic Homeland Intervention, Enforcement and Logistics Division assembles an elite covert team to find and deal with these threats wherever they are found. With a world rapidly becoming more bizarre and dangerous than ever before as the supervillains arise, these agents of S.H.I.E.L.D. are ready to take them on.